Affinity chromat

6-His human calretinin (recombinant) produced in Escherichia Coli。This recombinant protein has been purified by affinity chromatography on a NTA-resin This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection

Freeze in small aliquots (e.g. 1 or 10 µg) and keep at -80°C. Avoid repeated freezing and thawing

Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1993) Characterization of a polyclonal antiserum against the purified human recombinant calcium-binding protein calretinin. Cell Calcium 14: 639-648.