KILR® Retroparticles for Adherent Cells (Hygromycin B) contains MMLV particles, that can transduce a wide variety of adherent cells, resulting in high level expression with a housekeeping gene tagged with β-gal reporter fragment, ProLabel® (ePL). This results in a high level of expression of the fusion protein inside the target cells. Target cell death in a cytotoxicity assay results in the release of the ePL-tagged protein into the medium. Addition of KILR detection reagent, containing the complementing β-gal reporter fragment, Enzyme Acceptor (EA), results in complementation of the two enzyme fragments (EA and ePL). The resulting functional enzyme hydrolyzes a substrate to generate a chemiluminescent signal.

Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.