Catalogue number
PRO-2150
Synonyms
BCL2/Adenovirus E1B 19kDa Interacting Protein 1, NIP1, BCL2/Adenovirus
E1B 19 KDa Protein-Interacting Protein 1, Transformation-Related Gene 8
Protein, TRG-8, BCL2/Adenovirus E1B 19kD-Interacting Protein 1, Vesicle
Transport Protein SEC20, SEC20L, SEC20, Vesicle transport protein SEC20.
Introduction
BNIP1 belongs to the of the BCL2/adenovirus E1B 19 kd-interacting
protein (BNIP) family. BNIP1 interacts with the E1B 19 kDa protein, which defends
cells from virally-induced cell death. In addition, BNIP1 interacts with E1B 19
kDa-like sequences of BCL2 which is an additional apoptotic protector. Adding
up, BNIP1 is implicated in vesicle transport into the endoplasmic reticulum.
Alternative splicing results of BNIP1 in four protein products with identical
N- and C-termini have been found.
Description
BNIP1 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 222 amino acids (1-199 a.a) and having a molecular
mass of 25.2kDa.
BNIP1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
BNIP1
protein solution (1.0 mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAPQDV HVRICNQEIV KFDLEVKALI QDIRDCSGPL SALTELNTKV
KEKFQQLRHR IQDLEQLAKE QDKESEKQLL LQEVENHKKQ MLSNQASWRK ANLTCKIAID NLEKAELLQG
GDLLRQRKTT KESLAQTSST ITESLMGISR MMAQQVQQSE
EAMQSLVTSS RTILDANEEF KSMSGTIQLG RKLITKYNRR EL.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
