Catalogue number
PRO-2171
Synonyms
Secreted Frizzled-Related Protein 5, SARP3, Secreted Apoptosis Related Protein 3, Secreted Apoptosis-Related Protein 3, Frizzled-Related Protein 1b, FRP-1b, SARP-3, SFRP-5, FRP1B, SFRP5.
Introduction
Secreted Frizzled-Related Protein 5, also known as SFRP5, belongs to the
SFRP family which includes a cysteine-rich domain homologous to the putative
Wnt- of Frizzled proteins. SFRPs perform like soluble modulators
of Wnt signaling. SFRP5 & SFRP1 are
implicated in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the
retinal pigment epithelium, as well as moderately expressed in the pancreas.
Description
SFRP5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 311 amino acids (30-317 a.a) and having a molecular mass of 35kDa.
SFRP5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SFRP5 protein solution (1mg/ml) containing 20mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSEEYDYYG WQAEPLHGRS YSKPPQCLDI PADLPLCHTV GYKRMRLPNL LEHESLAEVK QQASSWLPLL AKRCHSDTQV FLCSLFAPVC LDRPIYPCRS LCEAVRAGCA PLMEAYGFPW PEMLHCHKFP LDNDLCIAVQ FGHLPATAPP VTKICAQCEM EHSADGLMEQ MCSSDFVVKM RIKEIKIENG DRKLIGAQKK KKLLKPGPLK RKDTKRLVLH MKNGAGCPCP QLDSLAGSFL VMGRKVDGQL LLMAVYRWDK KNKEMKFAVK FMFSYPCSLY YPFFYGAAEP H.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
