Catalogue number
ENZ-905
Synonyms
Aminoacylase-1, ACY-1, N-acyl-L-amino-acid amidohydrolase,
Aminoacylase 1, Acy1.
Introduction
Acy1
or Aminoacylase1 is a cytosolic, homodimeric, zinc-binding enzyme which
catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl
group, and has been suggested to operate in the catabolism and salvage of
acylated amino acids. ACY1 is localized
in chromosome 3p21.1, a region
reduced to homozygosity in small-cell lung cancer (SCLC), and its expression
has been observed to be reduced or undetectable in SCLC cell lines and tumors.
Description
ACY1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 433 amino acids (1-408 a.a) and having a molecular mass of 48.4kDa.
ACY1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
ACY1 protein solution (0.25mg/ml) containing Phosphate buffered saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMTTKD PESEHPSVTL FRQYLRICTV QPNPDYGGAI TFLEERARQL
GLSCQKIEVV PGFVITVLTW PGTNPSLPSI LLNSHTDVVP VFKEHWHHDP FEAFKDSEGY IYARGSQDMK SVSIQYLEAV
RRLKSEGHRF PRTIHMTFVP DEEVGGHKGM ELFVKRPEFQ ALRAGFALDE GLANPTDAFT VFYSERSPWW
VRVTSTGKPG HASRFIEDTA AEKLHKVISS ILAFREKERQ RLQANPHLKE GAVTSVNLTK LEGGVAYNVV
PATMSASFDF RVAPDVDMKA FEKQLQRWCQ EAGEGVTFEF AQKFTEPRMT PTDDSDPWWA AFSGACKAMN LTLEPEIFPA
ATDSRYIRAV GIPALGFSPM NRTPVLLHDH NERLHEDIFL RGVDIYTGLL SALASVPTLP GES.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
