Catalogue number
ENZ-704
Synonyms
Ribonuclease, RNase A Family, 7, Skin-Derived
Antimicrobial Protein 2, RNase 7, SAP-2,
EC 3.1.27.5, EC 3.1.27., EC 3.1.27, Ribonuclease 7.
Introduction
Ribonuclease 7 (RNASE7) is one of the final RNase A superfamily ribonucleases. RNASE7 was isolated from skin-derived stratum corneum. RNASE7 protein demonstrated potent ribonuclease activity and hence may contribute to the well-known ribonuclease activity of human skin. RNASE7 has revealed a broad spectrum antimicrobial activity against many pathogenic microorganisms and extraordinarily potent activity.
Description
RNASE7 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 151 amino acids (29-156 a.a) and
having a molecular mass of 16.9kDa.
RNASE7 is fused to a 23
amino acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
RNASE7 protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH8.0) and 10% glycerol 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSKPKGMTS SQWFKIQHMQ PSPQACNSAM KNINKHTKRC KDLNTFLHEP
FSSVAATCQT PKIACKNGDK NCHQSHGPVS LTMCKLTSGK YPNCRYKEKR QNKSYVVACK PPQKKDSQQF
HLVPVHLDRV L.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
