Catalogue number
ANT-033
Synonyms
NEU, NGL, HER2, TKR1, HER-2, c-erb B2, HER-2/neu, Receptor tyrosine-protein kinase erbB-2, EC 2.7.10.1, p185erbB2, C-erbB-2, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, MLN 19, CD340 antigen.
Type
Mouse Anti Human Monoclonal.
Introduction
HER-2/neu (erbB-2) encodes an 185-kDa orphan receptor tyrosine kinase that is constitutively active as a dimer and displays potent oncogenic activity when overexpressed. Herstatin, as the product of alternative HER-2 transcript, retains intron 8. The herstatin mRNA is expressed in normal human fetal kidney and liver, but is at reduced levels relative to p185HER-2 mRNA in carcinoma cells that contain an amplified HER-2 gene. Herstatin appears to be an inhibitor of p185HER-2, because it disrupts dimers, reduces tyrosine phosphorylation of p185, and inhibits the anchorage-independent growth of transformed cells that overexpress HER-2.
Clone
P7H3BV.
Immunogen
Anti-human ErbB2 mAb, is derived from mice immunized with the recombinant human ErbB2 (43.4 kDa, 397 a.a. and one extra N-terminal Methionin) purified from E. coli.
Antigen Amino Acid Sequence
MTQVCTGTDM KLRLPASPET HLDMLRHLYQ GCQVVQGNLE LTYLPTNASL SFLQDIQEVQ GYVLIAHNQV RQVPLQRLRI TQVCTGTDM KLRLPASPET HLDMLRHLYQ GCQVVQGNLE LTYLPTNASL SFLQDIQEVQ GYVLIAHNQV RQVPLQRLRI VRGTQLFEDN YALAVLDNGD PLNNTTPVTG ASPGGLRELQ LRSLTEILKG GVLIQRNPQL CYQDTILWKD IFHKNNQLAL TLIDTNRSRA CHPCSPMCKG SRCWGESSED CQSLTRTVCA GGCARCKGPL PTDCCHEQCA AGCTGPKHSD CLACLHFNHS GICELHCPAL VTYNTDTFES MPNPEGRYTF GASCVTACPY NYLSTDVGSC TLVCPLHNQE VTAEDGTQRC EKCSKPCARG THSLPPRPAA VPVPLRMQPG PAHPVLSFLR PSWDLVSAFY SLPLAPLSPT SVPISPVSVG RGPDPDAHVA VDLSRYEG.
Ig Subclass
IgG2b.
Species Reactivity
The ERBB2 antibody reacts with an epitope located on Human ERBB2 - without the last 79 amino acid residues.
Formulation
The ERBB2 antibody is lyophilized in 0.05M phosphate buffer, 0.1M NaCl, pH 7.2. AZIDE FREE.
Solubility
It is recommended to add 100µl of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which doesn't affect the activity of the ERBB2 antibody. The solution can be clarified by centrifugation.
Stability
The lyophilized antibody remains stable and fully active when stored at -20°C. It is recommended to aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. The Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Applications
ELISA, Western blotting.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
