Catalogue number
CYT-240
Synonyms
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Introduction
Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.
Description
Prolactin Ovine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23 kDa. The Prolactin n is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Solubility
It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.
Biological Activity
Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Safety Data Sheet
References
1.
Title:Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets.
Publication:Published online before print December 18, 2008, doi: 10.1210/en.2008-1318
Endocrinology April 1, 2009 vol. 150 no. 4 1646-1653 .
Link:http://endo.endojournals.org/content/150/4/1646.full
2.Title:ISLET ENDOTHELIAL CELLS AND PANCREATIC ?-CELL PROLIFERATION: STUDIES IN VITRO AND DURING PREGNANCY IN ADULT RATS.
Publication:Endocrinology. First published January 26, 2006 as doi:10.1210/en.2005-0997.
Link:http://endo.endojournals.org/content/early/2006/01/26/en.2005-0997.full.pdf
3.Title:Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets.
Publication:Endocrinology. First published ahead of print December 18, 2008 as doi:10.1210/en.2008-1318.
Link:http://endo.endojournals.org/content/early/2008/12/18/en.2008-1318.full.pdf
4. Title: Islet Endothelial Cells and Pancreatic -Cell
Proliferation: Studies in Vitro and during Pregnancy in Adult Rats.
Publication:Published online before print January 26, 2006, doi:10.1210/en.2005-0997 Endocrinology May 1, 2006vol. 147no. 52315-2324.
Link:http://endo.endojournals.org/content/147/5/2315.full.pdf+html
