Catalogue number
PRO-2154
Synonyms
Cell Growth Regulator With EF-Hand Domain 1, Cell Growth
Regulatory Gene 11 Protein, Hydrophobestin, CGR11, Cell Growth Regulator With
EF Hand Domain Protein 1, Cell Growth Regulator With EF Hand Domain 1, Cell
growth regulator with EF hand domain protein 1.
Introduction
Cell Growth Regulator with EF-Hand Domain 1, also known as CGREF1 is a
secreted calcium ion binding protein.
CGREF1 includes two EF-hand domains & both EF-hands are essential
for function. CGREF1 is most likely digested extracellularly by an unfamiliar
serine protease generating extremely hydrophobic bioactive peptides. CGREF1 mediates cell-cell
adhesion in a calcium-dependent manner. In addition, CGREF1 is capable to
inhibit growth in more than a few cell lines.
Description
CGREF1 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 305 amino acids (20-301 a.a) and having a
molecular mass of 32.3kDa.
CGREF1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
CGREF1 protein solution (0.5mg/ml) containing Phosphate
Buffered Saline (pH7.4).
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSAPKDGVT RPDSEVQHQL LPNPFQPGQE QLGLLQSYLK GLGRTEVQLE
HLSREQVLLY LFALHDYDQS GQLDGLELLS MLTAALAPGA ANSPTTNPVI LIVDKVLETQ DLNGDGLMTP
AELINFPGVA LRHVEPGEPL APSPQEPQAV GRQSLLAKSP LRQETQEAPG PREEAKGQVE ARRESLDPVQ
EPGGQAEADG DVPGPRGEAE GQAEAKGDAP GPRGEAGGQA EAEGDAPGPR GEAGGQAEAR ENGEEAKELP
GETLESKNTQ NDFEVHIVQV ENDEI.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
