Catalogue number
CYT-331
Synonyms
Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.
Introduction
FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
Description
Flt3-Ligand Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of approximately 17.6kDa.
Flt3-Ligand is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized with no additives.
Solubility
It is recommended to reconstitute the lyophilized Flt3-L in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-Ligand should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA.
Biological Activity
The ED50 as calculated by the dose-dependent stimulation of the proliferation of human AML5 cells is less than 1.0 ng/ml, corresponding to a Specific Activity of 1.0×106 IU/mg.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Safety Data Sheet
References
1.Title:Identification of Y589 and Y599 in the
juxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase SHP2.
Publication:Published online before printMay 9, 2006, doi:10.1182/blood-2005-07-008896
Submitted: July 14, 2005Accepted: April 26, 2006 BloodSeptember 1, 2006vol. 108no. 51542-1550.
Link:http://bloodjournal.hematologylibrary.org/content/108/5/1542.full
2. Title:A role of Gab2 association in Flt3 ITD mediated Stat5 phosphorylation and cell survival.
Publication:Received 2 February 2009; accepted for publication 30 March 2009 Correspondence: Lars Ro¨nnstrand,Experimental Clinical Chemistry, WallenbergLaboratory, Entr. 46, 5th fl, Malmo¨ University Hospital, SE-20502 Malmo¨, Sweden.E-mail: lars.ronnstrand@med.lu.se;http://www.expklkemi.mas.lu.se
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2141.2009.07725.x/pdf
