Catalogue number
ENZ-946
Synonyms
EGF domain-specific O-linked N-acetylglucosamine
transferase, Extracellular O-linked N-acetylglucosamine transferase.
Introduction
EGF
Domain-Specific O-Linked N-Acetylglucosamine Transferase (EOGT) takes part in
the regulation of Notch receptor. EOGT catalyzes
the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine/ threonine
residue in extracellular proteins resulting in their modification with a beta-linked
N-acetylglucosamine (O-GlcNAc). EOGT mainly
glycosylates the Thr residue positioned between the fifth and sixth conserved
cysteines of folded EGF-like domains.
Description
EOGT produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 516 amino acids (20-527 a.a.) and
having a molecular mass of 60.4kDa (Migrates at 50-70kDa on SDS-PAGE under
reducing conditions).
EOGT is expressed with an 8 amino acid His tag at C-Terminus
and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
EOGT
protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10%
glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 85.0% as determined by SDS-PAGE.
Amino acid sequence
DKAHSEADDA PGKALYDYSS LRLPAEHIPF FLHNNRHVAS VCREDSHCPY
KKHLENLNYC WGYEKSCAPE FRFGSPVCSY VDLGWTDTLE SAQDMFWRQA DFGYARERLG EIRTICQPER
ASDSSLVCSR YLQYCRATGL YLDLRNIKRN HDRFKEDFLQ GGEIGGYCKL DSHALVSEGQ RKSPLQSWFA
ELQGYTQLNF RPIEDAKCDI VVEKPTYFMK LDAGINMYHH FCDFLNLYLT QHVNNSFSTD VYIVMWDTST
YGYGDLFSDT WKAFTDYDVI HLKTYDSKKV CFKEAVFSLL PRMRYGLFYN TPLISGCQNT GLFRAFSQHV
LHRLNITQEG PKDGKVRVTI LARSTEYRKI LNQDELVNAL KTVSTFEVRV VDYKYRELGF LDQLRITHNT
DIFIGMHGAG LTHLLFLPDW AAVFELYNCE DERCYLDLAR LRGIHYITWR KPSKVFPQDK GHHPTLGEHP
KFTNYSFDVE EFMYLVLQAA EHVLQHPQWP FKKKHDELLE HHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
