Catalogue number
ENZ-693
Synonyms
Ribosomal Protein L12, L12, 60S ribosomal protein L12.
Introduction
Ribosomes, the organelles which catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are comprised of four RNA species and approximately 80 structurally distinct proteins. Ribosomal Protein L12 (RPL12) protein is a member of the L11P family of ribosomal proteins. RPL12 is a component of the 60S subunit, which is located in the cytoplasm. RPL12 binds directly to the 26S rRNA.
Description
RPL12 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 188 amino acids (1-165 a.a) and
having a molecular mass of 20.2kDa.
RPL12 is fused to a 23
amino acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
RPL12
protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M
NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSMPPKFDP
NEIKVVYLRC TGGEVGATSA LAPKIGPLGL SPKKVGDDIA KATGDWKGLR ITVKLTIQNR QAQIEVVPSA
SALIIKALKE PPRDRKKQKN IKHSGNITFD EIVNIARQMR HRSLARELSG TIKEILGTAQ SVGCNVDGRH
PHDIIDDINS GAVECPAS.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
