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Biotin Anti-Human IgD Rabbit Monoclonal Antibody, Clone RM123
品牌:RevMAb
货号:31-1025-02
规格:50 ug
货期:电询

商品详情 参考文献 相关资料

Product Details
Product Name Biotin Anti-Human IgD Rabbit Monoclonal Antibody [RM123]
Product Description Biotinylated Rabbit monoclonal to Human IgD
Catalog No. 31-1025-02
Clone Name RM123
Product Data Sheet Biotin Anti-Human IgD Rabbit Monoclonal Antibody Data Sheet RM123
Specificity RM123 reacts to human IgD. No cross reactivity with human IgG, IgM, IgE, or IgA.
Immunogen Human IgD
Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 25ng/well – 200ng/well (for Capture);
0.01ug/mL – 0.1ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
Immunohistochemistry

Immunohistochemistry of Human Lymphoid Tissue using
Anti-Human IgD antibody RM123.

Sandwich

Sandwich ELISA using RM123 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

ELISA

ELISA of human immunoglobulins shows RM123
reacts to IgD from human plasma and IgDκ from
human myeloma. No cross reactivity with human
IgG, IgM, IgA, or IgE. The plate was coated with
50 ng/well of different immunoglobulins. 200 ng/mL,
50ng/mL, or 10 ng/mL of RM123 was used as the
primary antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.

A

A titer ELISA using RM123. The plate was coated
with different amounts of human IgD (from plasma).
A serial dilution of RM123 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.


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