品牌：Emfret

货号：M042-1

品名：Emfret FITC-labeled Rat Anti-Mouse GPIbα (CD42b)  

规格：1.5 ml / 300 tests

克隆：Xia.G7 / Rat (Wistar) IgG1

包含：FITC-labeled immunoglobulin in 20 mM Tris buffer with 137 mM NaCl, 0.5% BSA and 0.09% (w/v) sodium azide

特异性：

The Xia.G7 antibody reacts with mouse GPIbα (CD42b), a platelet/megakaryocytespecific 150 kDa polypeptide that is disulfide-linked with GPIbβ (24 kDa) in the membrane. GPIb is part of the GPIb-V-IX complex, the platelet receptor for von Willebrand factor (vWf)1,2. Xia.G7 binds to the 130 kDa extracellular domain of GPIbα 3 , which is proteolytically released during platelet activation. Xia.G7 recognizes the membrane bound and the soluble form of GPIbα 3 .

用途：

 The antibody preparation is optimized for flow cytometric applications: Use 5 µl to stain ~106 platelets or ~0.5 x 106 cells in a recommended volume of 25 µl. Incubate for 15 minutes at room temperature, stop reaction by addition of 400 µl PBS and analyze samples within 30 minutes. For immunofluorescent staining of acetone-fixed frozen sections, the appropriate dilution must be determined individually. 

制备和储存：

The antibody was purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography. The antibody was conjugated with FITC under optimum conditions. The solution is free of unbound FITC. Store product undiluted at 4°C and avoid prolonged exposure to light. Stable for one year from date of shipment. Do not freeze. 

注意：

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer.

注：仅供研究使用，不用于诊断或治疗用途。