品牌：Emfret

货号：M042-0

品名：Emfret Purified Rat Anti-Mouse GPIba (CD42b)

规格：0.5 mg/ml 

克隆：Xia.G7 / Rat (Wistar) IgG1 

包含：0.5 mg purified immunoglobulin in PBS and 0.09% (w/v) sodium azide

特异性：

The Xia.G7 antibody reacts with mouse GPIba (CD42b), a platelet/megakaryocytespecific 150 kDa polypeptide that is disulfide-linked with GPIbb (24 kDa) in the membrane. GPIb is part of the GPIb-V-IX complex, the platelet receptor for von Willebrand factor (vWf)1,2. Xia.G7 binds to the 130 kDa extracellular domain of GPIba 3 (glycocalicin), which is proteolytically released during platelet activation. Xia.G7 recognizes the membrane-bound and the soluble form of GPIba 3 .

用途：

This preparation can be used for immunoprecipitation, immunohistochemical analysis of acetone-fixed frozen sections, and immunofluorescent staining (1-3 µg/106 platelets or cells). Xia.G7 recognizes mouse GPIba in a Western blot analysis under non-reducing condtions3 . For varying applications, appropriate dilutions must be determined individually.

制备和储存：

The antibody was purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography. Stable for six months from date of shipment when stored at 4°C. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.

注意：

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer.

注：仅供研究使用，不用于诊断或治疗用途。