品牌：Emfret

货号：X488

品名：Emfret Antibodies for In Vivo Mouse Platelet Labeling

规格：0.1 mg/ml 

同种型：Rat IgG (Wistar), derivatized

包含：100 µg DyLight488-labeled immunoglobulin derivative in phosphate buffered saline containing 0.2% BSA

特异性：

This antibody preparation contains a rat IgG derivative against the GPIbβ subunit of the murine platelet/megakaryocyte-specific GPIb-V-IX complex. The modified antibody has been optimized for the easy and stable in vivo labeling of circulating platelets in mice. At the recommended concentration (0.1 µg/g body weight), X488 is non-cytotoxic and does not interfere with platelet adhesion and aggregation in vivo. Also, X488 does not alter platelet adhesion on collagen/von Willebrand factor in vitro.1 In vivo platelet labeling has been used for intravital microscopical analysis of platelet involvement in pathological processes, such as thrombosis.2,3  

用途：

This preparation is optimized for rapid and stable in vivo labeling of mouse platelets. Use 0.1 µg (1 µl) X488 per gram body weight in an appropriate volume (50–200 µl) of sterile PBS for i.v. injection. Platelets can be visualized using FITC-fluorescence filter sets and a light-sensitive camera. Recommended exposure time: 200 – 400 ms.

制备和储存：

The antibodies were purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography and were biochemically modified. Stable for six months from date of shipment when stored at 4°C in the dark. KE EP STERILE, the preparation contains no preservative. 

注：仅供研究使用，不用于诊断或治疗用途。