品牌：Emfret

货号：P150-1

品名：Emfret FITC-labeled Rabbit Anti-von Willebrand factor (vWf)

规格：1.5 ml / 300 tests 

同种型：Rabbit polyclonal IgG

包含：FITC-labeled immunoglobulin in 20 mM Tris buffer with 137 mM NaCl, 0.5% BSA and 0.09% (w/v) sodium azide

特异性：

The polyclonal antibody reacts with murine and human von Willebrand factor (vWf), a multimeric plasma protein that is stored in α-granules of platelets and Weibel-Palade bodies of endothelial cells1 . The major receptors for vWf are GPIbα (CD42b) and activated integrin αIIbβ3 (CD41/61). GPIbα-vWf interaction can be induced by the snake venom protein botrocetin2 .

用途：

The antibody preparation is optimized for flow cytometric applications: Use 5 µl to stain ~106 platelets or ~0.5x106 cells in a recommended volume of 25 µl. Incubate for 15 minutes at room temperature, stop reaction by addition of 400 µl PBS and analyse samples within 30 minutes. For immunofluorescent staining of acetone-fixed frozen sections, the appropriate dilution must be determined individually. 

制备和储存：

The immunoglobulin was conjugated with FITC under optimum conditions. The solution is free of unbound FITC. Store product undiluted at 4°C and avoid prolonged exposure to light. Stable for one year from date of shipment. Do not freeze. 

注：仅供研究使用，不用于诊断或治疗用途。