Ready-to-use colorimetric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on caspase-8 cleavage site in CPP32/Caspase-3 proenzyme. FLICE and related caspase activity can be quantified by spectrophotometric detection of free pNA (l= 400 nm) after cleavage from the peptide substrate IETD-pNA, using a spectrophotometer or multi-well plate reader.

Additional Information

Synonyms	Ac-IETD-pNA, Caspase-8 Substrate, Colorimetric
Product #	C-2252
Chemical Name	Ac-IETD-pNA, Caspase-8 Substrate, Colorimetric
Formula	C27H38N6O12
MW	638.6
Appearance	Liquid
Purity	95% by HPLC
Storage Temp	-20°C
Use	Colorimetric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD

Additional Information

MDL Number	MFCD01318840
ChemACX	X1206333-3
InChI	InChI=1S/C27H38N6O12/c1-5-13(2)22(28-15(4)35)26(42)30-18(10-11-20(36)37)24(40)32-23(14(3)34)27(43)31-19(12-21(38)39)25(41)29-16-6-8-17(9-7-16)33(44)45/h6-9,13-14,18-19,22-23,34H,5,10-12H2,1-4H3,(H,28,35)(H,29,41)(H,30,42)(H,31,43)(H,32,40)(H,36,37)(H,38,39)/t13-,14+,18-,19-,22-,23-/m0/s1
SMILES	CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(=O)O)C(=O)Nc1ccc(cc1)[N+](=O)[O-])NC(=O)C
Peptide Sequence	AC-ILE-GLU-THR-ASP-PARANITROANILIDE