Details

Host: Mouse

Isotype: IgG

Class: Monoclonal

Immunogen: Synthetic peptide corresponding to DYKDDDDK (FLAG)

Applications:

Red-colored affinity matrix consists of affinity purified Anti-DYKDDDDK (FLAG) Tag (FG4R) monoclonal antibody covalently attached to red beaded agarose (Separopore®). Suitable for purifying N-terminal, Met-N-terminal, C-terminal DYKDDDDK (FLAG) fusion proteins, 3x DYKDDDDK (FLAG) fusion proteins; greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-Flag Red-Separopore® 4B-CL is reliable tool for purifying N-terminal, Met-N-terminal, C-terminal DYKDDDDK (FLAG) fusion proteins, 3x DYKDDDDK (FLAG) fusion proteins.

Technical Specifications:

• Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
• Concentration: 25 mg Red Separopore ® per mL of buffer
• Particle size range: 52 – 165 µm 
• Ligand coupling method: CNBr activation 
• Binding capacity: approximately ~600 µg/ml drained gel 
• Exclusion limit: approximately 2 x 10⁷
• pH stability: 3 – 9 (for use), 2 – 10 (for cleaning) 
• Supplied as 50% suspension in PBS, containing 50% glycerol. 

Additional Information

Additional Information