Description

Recombinant Amyloid-Beta-Peptide (1-40) uniformly 15N labeled

Amount

1mg

Format

Lyophilized

Sequence

DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV

Purity

95% by Chromatography and SDS-PAGE

Counter Ion

Ammonium Acetate

Solubility

Alexotech AB has developed a proprietary technique of preparing the amyloid β-peptide (1-40) having a superior solubility. It is however, of utmost importance to follow our recommendations of solubilisation: To efficiently solubilise the amyloid β-peptide (1-40) the pH should briefly be raised to between 11-12. This can be accomplished by 20 mM of NaOH, however, at high peptide concentrations a higher NaOH concentration may be required due to the intrinsic buffering capacity of the peptide. The pH should therefore always be monitored and if necessary adjusted. After solubilisation the pH can be adjusted using the buffer of choice.

Storage

Store at -20°C upon arrival.

Source

Protein expressed in Escherichia coli using 15NH4-Cl as sole nitrogen source.

Product Citations

Olofsson, A., Lindhagen-Persson, M., Vestling, M., Sauer-Eriksson, A. E., & Öhman, A. (2009). Quenched hydrogen/deuterium exchange NMR characterization of amyloid-β peptide aggregates formed in the presence of Cu2+or Zn2+. FEBS Journal, 276(15), 4051–4060. https://doi.org/10.1111/j.1742-4658.2009.07113.x