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ViroStat Immunochemicals for Infectious Disease

..... ViroStat Immunochemicals  for Infectious Disease

www.ViroStat-Inc.com

™ MONOTOPE product information for Yellow Fever virus
I. Monoclonal Antibody (Mouse) Specificity Product No.'s 3576 NA Specific for Yellow Fever virus. Negative for other Flaviviruses. ELISA
II. Purified Preparations Product No.'s 3576
      MONOTOPE™ purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations. The final preparation is formulated to a protein concentration of 100 µg/ml in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Each vial contains 1.0 ml. This product contains
THESE PRODUCTS ARE FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES NAIII. Fluorescein Conjugates Product No.'s
     These MONOTOPE™  products consist of purified monoclonal antibody conjugated with high purity isomer I of  fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The final preparation is formulated to an antibody concentration of of 100 µg/ml in 0.01 M phosphate buffered saline, pH 7.2 containing 0.1% sodium azide plus bovine serum albumin at 10 mg/ml.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES Comments:
IgG2a Ig class
no stabilizing proteins and should be stored at 2-8°C until ready for use.
Each vial contains 1.0 ml. This product should be stored at 2-8°C until ready for use. Avoid repeated freeze-thawing by storing multiple
Working dilution must be determined by the user. Suggested starting ranges are 1:10-1:50 for IFA  and 1:20-1:200 for ELISA.
aliquots at -20°C. Applications for these products include direct FA staining of target antigen in a permissive tissue culture system. Working strength must be determined by the user for each specific application but a starting range of 1:5 - 1:20 is recommended. Acetone fixation of the antigen source is recommended prior to staining.
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