accura-expRACE KIT 促销

◊In RACE (Rapid Amplification of cDNA Ends) by the single-primer method* of this kit, the targeted cDNA is amplified by PCR with only a gene-specific primer using ds cDNA as a template. 
◊The mechanism is based on that the terminal region of the ds cDNA is partially denatured at 68 C for the extension reaction and that the linear DNA molecule tends to circularize.
◊Upon reaching the 5’ end of the template DNA, thermostable DNA polymerase switches templates to the 5' terminal region of the newly synthesized daughter strand at a certain probability and synthesizes DNA sequences complementary to the gene-specific primer.
◊Using this daughter strand as a template, the targeted cDNA is amplified with only a gene- specific primer.

*US patent #7504240
Japanese patent #4304350

♦By using the accura-expRACE KIT, both 5’ and 3’ RACE can easily and efficiently be performed under simple conditions, such as RT-PCR.
♦ Long cDNA and rare cDNA unidentified previously can be isolated.
♦ The synthesized ds cDNA can be used as a cDNA library. You can perform ~400 screenings of a cDNA library by RACE.
♦ The cDNA synthesized by this kit contains a high proportion of full-length cDNA because an “advanced type M-MLV Reverse Transcriptase” is used.