BioAimScientific 1010010 人基质金属蛋白酶-9快速ELISA试剂盒 人基质金属蛋白酶-9试剂盒 Human MMP-9 EasyTest™ ELISA Kit
Human MMP-9 EasyTest™ ELISA KitHomeELISAEasyTest™ ELISA KitsHuman MMP-9 EasyTest™ ELISA Kit
Human MMP-9 EasyTest™ ELISA Kit
Human MMP-9 ELISA Kit Standard Curve
Human MMP-9 EasyTest™ ELISA Kit
Catalog No : 1010010
Sizes
$529.00
Product Features
Product Name Human MMP-9 EasyTest™ ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Process One wash step, two incubations
Assay Length 1.5 hours
Sample Volume Serum , Plasma, 50µL
Detection Method Colorimetric
Sensitivity 22pg/mL
Assay Range 65.5-16000pg/mL
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names MMP9; CLG4B; GELB; MANDP2; MMP-9
Gene ID 4318
Swissprot ID P14780
Recovery
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Serum
|
90
|
79-106
|
|
Plasma
|
92
|
90-100
|
Linearity
|
Sample Type
|
Dilution
|
% of Expected
|
|
Serum
|
1:2
|
118
|
|
1:4
|
94
|
|
1:8
|
88
|
|
Plasma
|
1:2
|
116
|
|
1:4
|
98
|
|
1:8
|
103
|
Standard Curve: Human MMP-9 EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human MMP-9 mix
• TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.
Background
Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix. In humans the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, angiogenesis, bone development, wound healing, cell migration, learning and memory, as well as in pathological processes, such as arthritis, intracerebral hemorrhage, and metastasis.
Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate interaction with matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human MMP-9 EasyTest™ ELISA Kit is for Research Use Only.
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