bioaimscientific 1010004 人骨形态发生蛋白-2快速ELISA试剂盒 人骨形态发生蛋白-2试剂盒 Human BMP-2 EasyTest™ ELISA Kit
Human BMP-2 EasyTest™ ELISA KitHomeELISAEasyTest™ ELISA KitsHuman BMP-2 EasyTest™ ELISA Kit
Human BMP-2 EasyTest™ ELISA Kit
Human BMP-2 ELISA Kit Standard Curve
Human BMP-2 EasyTest™ ELISA Kit
Catalog No : 1010004
Sizes
$529.00
Product Features
Product Name Human BMP-2 EasyTest™ ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Process One wash step, two incubations
Assay Length 2.0 hours
Sample Volume Serum , Plasma, 50µL
Detection Method Colorimetric
Sensitivity 25pg/mL
Assay Range 250-16000pg/mL
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names BMP2; BDA2A; BMP2
Gene ID 650
Swissprot ID P12643
Recovery
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Serum
|
105
|
90-120
|
|
Plasma
|
83
|
79-85
|
Linearity
|
Sample Type
|
Dilution
|
% of Expected
|
|
Serum
|
1:2
|
96
|
|
1:4
|
77
|
|
1:8
|
75
|
|
Plasma
|
1:2
|
113
|
|
1:4
|
111
|
|
1:8
|
97
|
Standard Curve: Human BMP-2 EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human BMP-2 mix
• TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of Bioaim mix.
4. Incubate 1.5 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.
Background
Bone morphogenetic protein 2 or BMP-2 belongs to the TGF-β superfamily of proteins. BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. BMP-2 and BMP-7 are osteoinductive BMPs: they have been demonstrated to potently induce osteoblast differentiation in a variety of cell types.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human BMP-2 EasyTest™ ELISA Kit is for Research Use Only.
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