Catalogue number
ENZ-966
Synonyms
Progastricsin (Pepsinogen C), Pepsinogen C, EC 3.4.23.3,
Pepsinogen Group II, Preprogastricsin, EC 3.4.23, Pepsin C, PGII, PEPC.
Introduction
Progastricsin-C
(PGC) is an aspartic proteinase which is synthesized in the gastric mucosa as
inactive precursors. PGC is a part of the peptidase family A1 and contains a
prosegment which is responsible for stabilizing the inactive form and preventing
the entrance of the substrate to the active site. PGC is used as a biomarker
for various gastric diseases including Helicobacter pylori related gastritis. PGC
is also hydrolyzes various proteins.
Description
PGC produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 380 amino acids (17-388 a.a.) and
having a molecular mass of 41.6kDa (Molecular size on SDS-PAGE will appear at
approximately 40-57kDa).
PGC is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
PGC protein solution (0.25mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
AVVKVPLKKF KSIRETMKEK GLLGEFLRTH KYDPAWKYRF GDLSVTYEPM
AYMDAAYFGE ISIGTPPQNF LVLFDTGSSN LWVPSVYCQS QACTSHSRFN PSESSTYSTN GQTFSLQYGS
GSLTGFFGYD TLTVQSIQVP NQEFGLSENE PGTNFVYAQF DGIMGLAYPA LSVDEATTAM QGMVQEGALT
SPVFSVYLSN QQGSSGGAVV FGGVDSSLYT GQIYWAPVTQ ELYWQIGIEE FLIGGQASGW CSEGCQAIVD
TGTSLLTVPQ QYMSALLQAT GAQEDEYGQF LVNCNSIQNL PSLTFIINGV EFPLPPSSYI LSNNGYCTVG
VEPTYLSSQN GQPLWILGDV FLRSYYSVYD LGNNRVGFAT AALEHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
