Catalogue number
ENZ-065
Synonyms
TAF9 RNA polymerase II TATA box binding protein (TBP)-associated factor 32kDa, TAFII32, AK6, CINAP, TAFII31, TAF2G, CGI-137, MGC1603, MGC3647, MGC5067, TAFIID32, Coilin-interacting nuclear ATPase protein, Transcription initiation factor TFIID 31/32 kDa subunit, STAF31/32, ATP-AMP transphosphorylase 6, h RNA polymerase II TBP-associated factor subunit G, CINAP, AD-004, CIP, adenylate kinase isoenzyme 6, adrenal gland protein AD-004, coilin interacting protein, MGC:1603, MGC:3647, MGC:5067, EC 2.7.4.3.
Introduction
TAF9 is a universal transcription factor which enables the preinitiation complex assembly by direct interactions with the TATA promoter element. TAF9 is a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs) and performs as a channel for regulatory signals.
Description
TAF9 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 192 amino acids (1-172.a.a) and having a molecular mass of 22.2kDa.
TAF9 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
TAF9 Human solution (0.5mg/1ml) containing 20mM Trsi HCL pH-8, 1mM DTT and 10% glycerol.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MLLPNILLTG TPGVGKTTLG KELASKSGLK YINVGDLARE EQLYDGYDEE YDCPILDEDR VVDELDNQMR EGGVIVDYHG CDFFPERWFH IVFVLRTDTN VLYERLETRG YNEKKLTDNI QCEIFQVLYE EATASYKEEI VHQLPSNKPE ELENNVDQIL KWIEQWIKDH NS
Purity
Greater than 90% as determined by SDS-PAGE.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
