Catalogue number
ENZ-936
Synonyms
TIMP
Metallopeptidase Inhibitor 2, Tissue Inhibitor Of Metalloproteinases 2, CSC-21K,
Tissue Inhibitor Of Metalloproteinase 2, TIMP-2, DDC8, Metalloproteinase
inhibitor 2.
Introduction
TIMP2 belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases that take part in degradation of the extracellular matrix. Besides having an inhibitory role against metalloproteinases, the encoded protein has a exclusive part among TIMP family members in its capability to directly suppress the proliferation of endothelial cells. Consequently, the encoded protein is crucial to the conservation of tissue homeostasis by suppressing the production of quiescent tissues as an answer to angiogenic factors, and by inhibiting protease activity in tissues undergoing renovation of the extracellular matrix.
Description
TIMP2 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 200 amino acids (27-220a.a.) and
having a molecular mass of 22.5kDa.
(Molecular size on SDS-PAGE will appear at
approximately 18-28kDa).
TIMP2 is expressed with a 6
amino acid His tag at C-Terminus and purified by proprietary chromatographic
techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
TIMP2 protein solution (0.25mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4
weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw
cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI
KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH
RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG
AAPPKQEFLD IEDPHHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
