Catalogue number
ENZ-271
Synonyms
Matrilysin, EC 3.4.24.23, Pump-1 protease, Uterine metalloproteinase, Matrix metalloproteinase-7, MMP-7, Matrin, MPSL1, PUMP-1, MMP7.
Introduction
Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.
Description
Matrix Metalloproteinase-7 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 19.13 kDa.
The MMP-7 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile clear liquid solution.
Formulation
The protein contains the following additives 10mM HEPES (pH 7.4), 5mM CaCl2 and 150mM NaCl.
Stability
Matrilysin although stable at 4°C for 3 weeks, should be stored below -18°C.
Please prevent freeze-thaw cycles.
Biological Activity
The specific activity was found to be 6,200 U/mg.
Unit Definition
One unit is defined as the digestion of 1 µg Azocoll/min at 37°C.
Applications
Western Blotting (1:1K-5K for neat serum and 1-10 ug/ml for affinity pure antibody using ECL technique).
ELISA: Control peptide can be used to coat ELISA plates at 1 ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1 ug/ml for affinity pure).
Histochemistry & Immunofluorescence: Not tested.
References
Title: Interaction of Periosteal Explants with Articular Chondrocytes AltersExpression Pro?le of Matrix Metalloproteinases
Publication: Journal of Orthopaedic Research 28.12 (2010): 1576-1585.
Link:http://onlinelibrary.wiley.com/doi/10.1002/jor.21154/epdf
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
