Catalogue number
PRO-261
Synonyms
Proteasome assembly chaperone 3, PAC-3, hPAC3, PSMG3, PAC3, C7orf48, MGC10911.
Introduction
PSMG3 is a chaperone protein that promotes assembly of the 20S proteasome. PSMG3 may cooperate with PSMG1-PSMG2 heterodimers to coordinate the correct assembly of proteasomes. PSMG3 interacts with PSMG4, as well as directly with alpha and beta subunits of the 20S proteasome but dissociates prior to the formation of half-proteasomes, probably upon recruitment of POMP.
Description
PSMG3 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 142 amino acids (1-122 a.a.) and having a molecular mass of 15.2kDa. The PSMG3 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The PSMG3 solution (0.5 mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0) and 10% Glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MEDTPLVISK QKTEVVCGVP TQVVCTAFSS HILVVVTQFG KMGTLVSLEP SSVASDVSKP VLTTKVLLGQ DEPLIHVFAK NLVAFVSQEA GNRAVLLAVA VKDKSMEGLK ALREVIRVCQ VW.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
