Catalogue number
ENZ-865
Synonyms
Acid
Phosphatase 6, Lysophosphatidic, Acid Phosphatase-Like Protein 1, PACPL1,
ACPL1, LPAP, Lysophosphatidic Acid Phosphatase Type 6, Lysophosphatidic Acid
Phosphatase 6, Acid Phosphatase Like 1, EC 3.1.3.2, Lysophosphatidic acid
phosphatase type 6.
Introduction
Acid Phosphatase-6, also known as ACP6 is Hydrolyzes lysophosphatidic
acid (LPA) which contains a medium length fatty acid chain to the corresponding
monoacylglycerol. ACP6 shows highest activity with lysophosphatidic acid which
contains myristate (C14:0), monounsaturated oleate (C18:1) or palmitate (C16:0),
and lower activity with C18:0 as well as C6:0 lysophosphatidic acid.
Description
ACP6 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 419 amino acids (33-428a.a) and
having a molecular mass of 47.7kDa.
ACP6 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
ACP6 protein solution (1mg/ml) containing Phosphate
buffered saline (pH7.4), 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSELQEADG QCPVDRSLLK LKMVQVVFRH GARSPLKPLP LEEQVEWNPQ
LLEVPPQTQF DYTVTNLAGG PKPYSPYDSQ YHETTLKGGM FAGQLTKVGM QQMFALGERL RKNYVEDIPF
LSPTFNPQEV FIRSTNIFRN LESTRCLLAG LFQCQKEGPI
IIHTDEADSE VLYPNYQSCW SLRQRTRGRR QTASLQPGIS EDLKKVKDRM
GIDSSDKVDF FILLDNVAAE QAHNLPSCPM LKRFARMIEQ RAVDTSLYIL PKEDRESLQM AVGPFLHILE
SNLLKAMDSA TAPDKIRKLY LYAAHDVTFI PLLMTLGIFD HKWPPFAVDL TMELYQHLES
KEWFVQLYYH GKEQVPRGCP
DGLCPLDMFL NAMSVYTLSP EKYHALCSQT QVMEVGNEE.
Biological Activity
Specific activity is > 70 units/mg, and is defined as the amount of enzyme which hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at PH 5.0 at 37C.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
