Catalogue number
PRO-2334
Synonyms
Stress Induced Phosphoprotein 1, Transformation-Sensitive
Protein IEF SSP 3521, Renal Carcinoma Antigen NY-REN-11,
Stress-Induced-Phosphoprotein 1, Hsp70/Hsp90-Organizing Protein,
Hsc70/Hsp90-Organizing Protein, STI1, HOP, Epididymis Secretory Sperm Binding
Protein Li 94n, NY-REN-11 Antigen, IEF-SSP-3521, HEL-S-94n, STI1L, P60.
Introduction
STIP1 is an adaptor protein that mediates the functions of HSP70 & HSP90 in protein folding. STIP1 supports the transfer of proteins from HSP70 to HSP90 by binding together HSP90 and substrate-bound HSP70. STIP1 stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones. STIP1 genetic variations are involved in regulating corticosteroid response in asthmatic subjects with reduced lung function.
Description
STIP1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 543 amino acids (1-543 a.a) and
having a molecular mass of 62.6kDa.
STIP1 is purified by proprietary chromatographic
techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
STIP1 protein solution (0.5mg/ml) containing 20mM
Tris-HCl (pH 8.0), 10% glycerol, 1mM DTT and 0.1M NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4
weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MEQVNELKEK GNKALSVGNI DDALQCYSEA IKLDPHNHVL YSNRSAAYAK
KGDYQKAYED GCKTVDLKPD WGKGYSRKAA ALEFLNRFEE AKRTYEEGLK HEANNPQLKE GLQNMEARLA
ERKFMNPFNM PNLYQKLESD PRTRTLLSDP TYRELIEQLR NKPSDLGTKL QDPRIMTTLS VLLGVDLGSM
DEEEEIATPP PPPPPKKETK PEPMEEDLPE NKKQALKEKE LGNDAYKKKD FDTALKHYDK AKELDPTNMT
YITNQAAVYF EKGDYNKCRE LCEKAIEVGR ENREDYRQIA KAYARIGNSY FKEEKYKDAI HFYNKSLAEH
RTPDVLKKCQ QAEKILKEQE RLAYINPDLA LEEKNKGNEC FQKGDYPQAM KHYTEAIKRN PKDAKLYSNR
AACYTKLLEF QLALKDCEEC IQLEPTFIKG YTRKAAALEA MKDYTKAMDV YQKALDLDSS CKEAADGYQR
CMMAQYNRHD SPEDVKRRAM ADPEVQQIMS DPAMRLILEQ MQKDPQALSE HLKNPVIAQK IQKLMDVGLI AIR.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
