Catalogue number
PRO-2082
Synonyms
Selenoprotein W, 1, SelW, Selenoprotein W.
Introduction
Selenoprotein W 1, also known as SEPW1 is a selenoprotein, which has a
selenocysteine (Sec) residue at its active site. The selenocysteine is encoded
through the UGA codon which normally signals translation termination. The 3'
UTR of selenoprotein genes share a common stem-loop structure, the sec
insertion sequence (SECIS), which is essential for the recognition of UGA as a
Sec codon instead of as a stop signal. SEPW1 shows highest expression in
skeletal muscle and heart, and also involved in oxidation-reduction reactions.
Description
SEPW1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 110 amino acids (1-87 a.a) and
having a molecular mass of 11.8kDa.
SEPW1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SEPW1 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMALAVRV VYCGACGYKS KYLQLKKKLE DEFPGRLDIC GEGTPQATGF
FEVMVAGKLI HSKKKGDGYV DTESKFLKLV AAIKAALAQG.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
