Catalogue number
PRO-1334
Synonyms
REM2- and Rab-like small GTPase 1, RSG1, C1orf89, RP4-733M16.4.
Introduction
REM2 and RAB-Like Small GTPase 1 (RSG1) plays a part in targeted membrane trafficking most likely at the level of vesicle fusion with membranes. The RSG1 protein is involved in cilium biogenesis by regulating the transportation of cargo proteins to the basal body and to the apical tips of cilia. RSG1 is a potential effector of the planar cell polarity signaling pathway. RSG1 is also involved in exocytosis in secretory cells.
Description
RSG1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-258 a.a) and having a molecular mass of 30.9kDa.
RSG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
RSG1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.5), 0.2M NaCl, 50% glycerol and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMARPPVP GSVVVPNWHE SAEGKEYLAC ILRKNRRRVF GLLERPVLLP PVSIDTASYK IFVSGKSGVG KTALVAKLAG LEVPVVHHET TGIQTTVVFW PAKLQASSRV VMFRFEFWDC GESALKKFDH MLLACMENTD AFLFLFSFTD RASFEDLPGQ LARIAGEAPG VVRMVIGSKF DQYMHTDVPE RDLTAFRQAW ELPLLRVKSV PGRRLADGRT LDGRAGLADV AHILNGLAEQ LWHQDQVAAG LLPNPPESAP E.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
