Catalogue number
PRO-1467
Synonyms
DHLAG, HLADG, Ia-GAMMA, li, HLA-DR antigens-associated
invariant chain, p33, Ia antigen-associated invariant chain, HLA class II
histocompatibility antigen gamma chain, CD74, Gamma Chain Of Class II Antigens,
MHC HLA-DR Gamma Chain.
Introduction
CD74
(HLA class II histocompatibility antigen gamma chain isoform b), is a transmembrane
protein (on antigen presenting cells) which is the invariant chain that
chaperones MHC class II dimers from the endoplasmic
reticulum to the cell surface. CD74 is expressed by cells of both T lymphocyte
and B lymphocyte lineages. CD74 upregulated in several cancers
and is also expressed by non immune cells during inflammation. Diseases related with CD74 include thymic epithelial neoplasm and
gastroduodenitis. Among its related super-pathways are Immune System and Immune
response Antigen presentation by MHC class II. In addition CD74 functions as
cell surface receptor for the cytokine MIF.
Description
CD74 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 183 amino acids (73-232 a.a) and having a molecular mass of 20.6kDa.
CD74 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CD74 protein solution (0.5mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.1M NaCl, 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSQQQGRLD KLTVTSQNLQ LENLRMKLPK PPKPVSKMRM ATPLLMQALP MGALPQGPMQ
NATKYGNMTE DHVMHLLQNA DPLKVYPPLK GSFPENLRHL KNTMETIDWK VFESWMHHWL LFEMSRHSLE
QKPTDAPPKE SLELEDPSSG LGVTKQDLGP VPM
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
