Catalogue number
PRO-2066
Synonyms
Pellino E3 Ubiquitin Protein Ligase Family Member 2, Pellino-2,
E3 Ubiquitin-Protein Ligase Pellino Homolog 2, Pellino (Drosophila) Homolog 2, Pellino
Homolog 2 (Drosophila), Protein Pellino Homolog 2, Pellino Homolog 2, EC 6.3.2,
PELI2.
Introduction
Pellino E3 Ubiquitin Protein Ligase Family Member 2, also known as PELI2
is an E3 ubiquitin ligase which catalyzes the covalent attachment of ubiquitin
moieties onto substrate proteins. PELI2 is implicated in the TLR and IL-1
signaling pathways through interaction with the complex which contain IRAK
kinases and TRAF6. PELI2 also mediates IL1B-induced IRAK1 'Lys-63'-linked
polyubiquitination and possibly 'Lys-48'-linked ubiquitination. PELI2 is significant
for LPS- and IL1B-induced MAP3K7-dependent, however not for MAP3K3-dependent,
NF-kappa-B activation. Moreover, PELI2 can activate the MAP (mitogen activated
protein) kinase pathway directing to activation of ELK1.
Description
PELI2 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 443 amino acids (1-420 a.a) and
having a molecular mass of 48.8kDa.
PELI2 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
PELI2 protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMFSPGQE EHCAPNKEPV KYGELVVLGY NGALPNGDRG RRKSRFALYK
RPKANGVKPS TVHVISTPQA SKAISCKGQH SISYTLSRNQ TVVVEYTHDK DTDMFQVGRS TESPIDFVVT
DTISGSQNTD EAQITQSTIS RFACRIVCDR NEPYTARIFA AGFDSSKNIF LGEKAAKWKN PDGHMDGLTT NGVLVMHPRG GFTEESQPGV
WREISVCGDV YTLRETRSAQ QRGKLVESET NVLQDGSLID LCGATLLWRT ADGLFHTPTQ KHIEALRQEI
NAARPQCPVG LNTLAFPSIN RKEVVEEKQP WAYLSCGHVH GYHNWGHRSD TEANERECPM CRTVGPYVPL WLGCEAGFYV DAGPPTHAFT PCGHVCSEKS AKYWSQIPLP
HGTHAFHAAC PFCATQLVGE QNCIKLIFQG PID.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
