Catalogue number
ENZ-819
Synonyms
Phosphodiesterase 6H, CGMP-Specific, Cone, Gamma, Retinal
Cone Rhodopsin-Sensitive CGMP 3',5'-Cyclic Phosphodiesterase, Subunit Gamma, EC
3.1.4.35, EC 3.1.4.17, RCD3, GMP-PDE Gamma, ACHM6, PDE6H.
Introduction
PDE6H
belongs to the rod/cone cGMP-PDE gamma subunit family, which selectively
catalyze the hydrolysis of 3 cyclic phosphate bonds in adenosine and/or guanine
3,5 cyclic monophosphate (cAMP and/or cGMP). This family regulates the cellular
levels, localization and duration of action of these second messengers by
controlling the rate of their degradation. PDE6H is the inhibitory (or gamma)
subunit of the cone-specific cGMP phosphodiesterase, which is a tetramer
composed of two catalytic chains (alpha and beta), and two inhibitory chains (gamma).
PDE6H is particularly expressed in the retina, and is implicated in the
transmission and amplification of the visual signal. Mutations in PDE6H have
been associated with retinal cone dystrophy type 3A.
Description
PDE6H Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 106 amino acids (1-83 a.a) and
having a molecular mass of 11.5 kDa.
PDE6H is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
PDE6H protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 2mM DTT and 0.1mM PMSF.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSDNTTL PAPASNQGPT TPRKGPPKFK QRQTRQFKSK PPKKGVKGFG
DDIPGMEGLG TDITVICPWE AFSHLELHEL AQFGII.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
