Catalogue number
DEN-041
Introduction
Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholino antisense oligos have shown specific activity against Dengue virus.
Description
Dengue-gold is an antigen used for dengue antibody rapid test. This antigen is genetically developed from 4 dengue subtypes. This antigen can be recognized by most of the dengue antibodies from infected patients.
Dengue Antigen for Rapid Test has over 90% sensitivity and specificity over 90% to detect both dengue IgM and IgG.
Source
E.coli.
Purification Method
Purified by proprietary chromatographic technique.
Physical Appearance
Sterile Filtered clear solution.
Formulation
Phosphate buffered saline pH 7.4 and 0.02% sodium nitrate.
Stability
Recombinant Dengue Rapid Test although stable at 4C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Purity
Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Assay Conditions
1. Prepare 528 to 533nm colloid gold, conjugation within 24 hours after colloid gold preparation;
2. Adjust colloid gold OD at 1.3 to 1.4, conjugation concentration and pH are 20ug / ml gold and 7.2;
3. Shanking conjugation for 8-10 hours, then centrifuge the conjugation at 10,000 rpm at 20oC for 20 min;
4. Remove the supernatnant as more as possible, add the supplied gold suspension buffer to adjust OD to 8.2;
5. Add the supplied blocking reagent to the final concentration to 0.4%;
6. Blocking for 1 hours , then add sucrose to the final concentration to 20%;
7. Dipping the conjugated gold to the gold pad ( do not use sodium phosphate for gold pad treatment);
8. After drying, assemble gold pad and absorbent pad over backing card;
9. Add 10µl serum/plasma and add the supplied running buffer for assay.
10. It is suggested to use our monoclonal anti-human IgG and IgM cat# ant-529 and ant-530 for rapid test development kit.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
