Catalogue number
PKA-070
Synonyms
Cell Growth Regulator With EF-Hand Domain 1, Cell Growth
Regulatory Gene 11 Protein, Hydrophobestin, CGR11, Cell Growth Regulator With
EF Hand Domain Protein 1, Cell Growth Regulator With EF Hand Domain 1, Tribbles
homolog 3.
Introduction
Tribbles Pseudokinase 3, also known as TRIB3, is a putative protein
kinase which is induced by the transcription factor NF-kappaB. In addition,
TRIB3 is a negative regulator of NF-kappaB, and can as well sensitize cells to
TNF- and TRAIL-induced apoptosis. Furthermore, TRIB3 is able to negatively
regulate the cell survival serine-threonine kinase AKT1. One of the diseases
which are associated with TRIB3 is anoxia.
Description
TRIB3 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 381 amino acids (1-358 a.a) and
having a molecular mass of 42.0kDa.
TRIB3 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
TRIB3 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMRATPLA APAGSLSRKK RLELDDNLDT ERPVQKRARS GPQPRLPPCL
LPLSPPTAPD RATAVATASR LGPYVLLEPE EGGRAYQALH CPTGTEYTCK VYPVQEALAV LEPYARLPPH
KHVARPTEVL AGTQLLYAFF TRTHGDMHSL VRSRHRIPEP EAAVLFRQMA TALAHCHQHG LVLRDLKLCR
FVFADRERKK LVLENLEDSC VLTGPDDSLW DKHACPAYVG PEILSSRASY SGKAADVWSL GVALFTMLAG
HYPFQDSEPV LLFGKIRRGA YALPAGLSAP ARCLVRCLLR REPAERLTAT GILLHPWLRQ DPMPLAPTRS
HLWEAAQVVP DGLGLDEARE EEGDREVVLY G.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
