Catalogue number
PKA-322
Synonyms
Protein kinase C epsilon type, EC 2.7.11.13, nPKC-epsilon, PRKCE, PKCE, MGC125656, MGC125657.
Introduction
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.
Description
PRKCE Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-737 and having a molecular mass of 87 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag.
PRKCE is purified by proprietary chromatographic techniques.
Source
Sf9 insect cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
PRKCE is supplied in 20mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF, 1mM benzamidine and 0.03% Brij-35.
Unit Definition
3250 units/mg. One unit of PRKCE activity transfers 1 nmole of phosphate to a PRKCE substrate peptide per minute at 30°C. Recombinant human active PRKCE also phosphorylates full length myelin basic protein, MARKS, GAP-34, and raf-1 kinase. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
