Catalogue number
ENZ-914
Synonyms
PSMD5, Proteasome (Prosome, Macropain) 26S Subunit,
Non-ATPase, 526S Protease Subunit S5 Basic,26S Proteasome Subunit
S5B,S5B,26S Proteasome Non-ATPase Regulatory Subunit 5,KIAA0072.
Introduction
Proteasome
26S Subunit, Non-ATPase 5, also known as PSMD5 is a member of the proteasome
subunit S5B/HSM3 family. The 26S proteasome is an enzymatic complex which
degrades ubiquitinated proteins in eukaryotic cells. Furthermore, PSMD5 acts as
a chaperones which is involved in the assembly of the 26s proteasome, particularly
of the base subcomplex of the PA700/19S regulatory complex. PSMD5 is full of
dileucine repeats, which have been involved in trafficking of various transmembrane
proteins.
Description
PSMD5 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 529 amino acids (1-504 a.a) and
having a molecular mass of 58.9kDa.
PSMD5 is fused to a 25 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
PSMD5 protein solution (1mg/ml) containing Phosphate
Buffered Saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMAAQA LALLREVARL EAPLEELRAL HSVLQAVPLN ELRQQAAELR
LGPLFSLLNE NHREKTTLCV SILERLLQAM EPVHVARNLR VDLQRGLIHP DDSVKILTLS QIGRIVENSD
AVTEILNNAE LLKQIVYCIG GENLSVAKAA IKSLSRISLT QAGLEALFES NLLDDLKSVM KTNDIVRYRV
YELIIEISSV SPESLNYCTT SGLVTQLLRE LTGEDVLVRA TCIEMVTSLA YTHHGRQYLA QEGVIDQISN
IIVGADSDPF SSFYLPGFVK FFGNLAVMDS PQQICERYPI FVEKVFEMIE SQDPTMIGVA VDTVGILGSN
VEGKQVLQKT GTRFERLLMR IGHQSKNAPV
ELKIRCLDAI SSLLYLPPEQ QTDDLLRMTE SWFSSLSRDP LELFRGISSQ PFPELHCAAL KVFTAIANQP WAQKLMFNSP
GFVEYVVDRS VEHDKASKDA KYELVKALAN SKTIAEIFGN PNYLRLRTYL SEGPYYVKPV STTAVEGAE.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
