Catalogue number
ENZ-322
Synonyms
EC 3.1.3.1, IAP, AP, ALPI, Intestinal-type alkaline phosphatase, Intestinal alkaline phosphatase.
Description
The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
Source
Calf Intestine.
Physical Appearance
Sterile Filtered clear colorless solution.
Formulation
50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.
Stability
AP should be stored at 4°C.
Please Do-Not freeze.
Purity
95% pure by Gel Filtration.
Biological Activity
>1000 U/mg (pH 9.6, 25°C and 0.025M glycin, 10% glycerol as buffer.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
