Catalogue number
ENZ-967
Synonyms
Lactate Dehydrogenase B, Renal Carcinoma Antigen
NY-REN-46, LDH Heart Subunit, EC 1.1.1.27, LDH-B, LDH-H, Epididymis Secretory
Protein Li 281, Testicular Secretory Protein Li 25, Lactate Dehydrogenase H
Chain, HEL-S-281, EC 1.1.1 , LDHBD, TRG-5.
Introduction
LDHB is part of the lactate dehydrogenase family. LDHB is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. LDHB catalyzes the oxidation of hydroxybutyrate, and frequently called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors.
Description
LDHB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 334 amino acids (1-334) and having a molecular mass of 36.6kDa.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The LDHB solution (1mg/ml) contains 20mM Tris-HCl (pH 8.0), 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90% as determined by SDS-PAGE.
Biological Activity
Specific activity is >300 units/mg, in which 1 unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per min at pH 7.5 at 37°.
Amino acid sequence
MATLKEKLIA
PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA
DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK
LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG
TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL
SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
