Catalogue number
ENZ-279
Synonyms
Lactate Dehydrogenase, LDH.
Introduction
Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.
A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Description
The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.
Source
Escherichia Coli.
Physical Appearance
Sterile lyophilized powder.
Formulation
The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
Solubility
It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity
The specific activity was found to be 258 U/mg protein.
Unit Definition
One unit is defined as 1umol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include a-hydroxyglutarate dehydrogenase activity.
References
Title: UDP-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase (GNE)
Binds to Alpha-Actinin 1: Novel Pathways in Skeletal Muscle?
Publications: PLoS One 3.6 (2008): e2477.
Link: http://journals.plos.org/plosone/articleid=10.1371/journal.pone.0002477#s1
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
