Catalogue number
ENZ-037
Synonyms
Gpnat1, GNPNAT, GNA1, EC 2.3.1.4, FLJ10607, Glucosamine-Phosphate N-Acetyltransferase 1, Phosphoglucosamine acetylase, Phosphoglucosamine transacetylase, Glucosamine 6-Phosphate N-Acetyltransferase.
Introduction
GNPNAT1 is a member of the GNA1 subfamily of the larger acetyltransferase family of proteins. GNPNAT1 is limited to the Golgi apparatus and the endosome. GNPNAT1 is vital for UDPGlcNAc biosynthesis pathway. GNPNAT1 catalyzes the synthesis of GlcNAc6P from AcCoA and GlcN6P, a step in the UDP-GlcNAc6P formation pathway.
Description
GNPNAT1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (1-184a.a.) and having a molecular mass of 23.1KDa.
GNPNAT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The GNPNAT1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 30% glycerol.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMKPDETP MFDPSLLKEV DWSQNTATFS PAISPTHPGE GLVLRPLCTA DLNRGFFKVL GQLTETGVVS PEQFMKSFEH MKKSGDYYVT VVEDVTLGQI VATATLIIEH KFIHSCAKRG RVEDVVVSDE CRGKQLGKLL LSTLTLLSKK LNCYKITLEC
LPQNVGFYKK FGYTVSEENY MCRRFLK
Purity
Greater than 90% as determined by SDS-PAGE.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
