Catalogue number
PKA-071
Synonyms
Proto-Oncogene B-Raf, BRAF1, RAFB1, NS7, EC 2.7.11.1, B-RAF1, P94.
Introduction
B-Raf Proto-Oncogene, also known as BRAF is a member of the raf/mil
family of serine/threonine protein kinases. BRAF participates in regulating the
MAP kinase/ERKs signaling pathway, which eventually have an effect on cell
division, differentiation, and secretion. Mutations in BRAF have been
associated with cardiofaciocutaneous syndrome, which is a disease characterized
by heart defects, mental retardation and a distinctive facial appearance. In
addition, mutations in BRAF have also been associated with different cancers,
including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid
carcinoma, non-small cell lung carcinoma, as well as adenocarcinoma of lung.
Description
BRAF Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 360 amino acids (432-766a.a) and having a
molecular mass of 40.6kDa.
BRAF is fused to a 25 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
BRAF
protein solution (0.25mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSEFSEDRN RMKTLGRRDS SDDWEIPDGQ ITVGQRIGSG SFGTVYKGKW
HGDVAVKMLN VTAPTPQQLQ AFKNEVGVLR KTRHVNILLF MGYSTKPQLA IVTQWCEGSS LYHHLHIIET
KFEMIKLIDI ARQTAQGMDY LHAKSIIHRD LKSNNIFLHE DLTVKIGDFG LATVKSRWSG SHQFEQLSGS
ILWMAPEVIR MQDKNPYSFQ SDVYAFGIVL YELMTGQLPY SNINNRDQII FMVGRGYLSP DLSKVRSNCP
KAMKRLMAEC LKKKRDERPL FPQILASIEL LARSLPKIHR SASEPSLNRA GFQTEDFSLY ACASPKTPIQ
AGGYGAFPVH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
