Catalogue number
ENZ-849
Synonyms
Acid Phosphatase 2, Lysosoma, EC 3.1.3.2 LAP, Lysosomal Acid Phosphatase, ACP2.
Introduction
Acid Phosphatase-2, also
known as ACP2 is composed of two subunits, Alpha & beta, and is chemically as well as genetically distinct from
red cell acid phosphatase. ACP2 belongs to a family of distinct isoenzymes
which hydrolyze orthophosphoric monoesters to alcohol and phosphate. In
addition, Acid phosphatase deficiency is caused by mutations in the ACP2-beta subunit as well as
ACP3-alpha subunit genes.
Description
ACP2 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 373 amino acids (31-380 a.a.) and having a
molecular mass of 42.9kDa.
ACP2 is fused to a 23 amino acid His-Tag at N-Terminus
and purified by conventional chromatography techniques.Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
ACP2 protein solution (1mg/ml) contains 20mM Tris-HCl (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSRSLRFVT LLYRHGDRSP VKTYPKDPYQ EEEWPQGFGQ LTKEGMLQHW ELGQALRQRY HGFLNTSYHR QEVYVRSTDF DRTLMSAEAN LAGLFPPNGM QRFNPNISWQ PIPVHTVPIT EDRLLKFPLG PCPRYEQLQN ETRQTPEYQN ESSRNAQFLD MVANETGLTD LTLETVWNVY DTLFCEQTHG LRLPPWASPQ TMQRLSRLKD FSFRFLFGIY QQAEKARLQG GVLLAQIRKN LTLMATTSQL PKLLVYSAHD TTLVALQMAL DVYNGEQAPY ASCHIFELYQ EDSGNFSVEM YFRNESDKAP WPLSLPGCPH RCPLQDFLRL TEPVVPKDWQ QECQLASGPA DTE
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
