Catalogue number
ENZ-738
Synonyms
CATSF, CLN13, Cathepsin F, EC=3.4.22.41.
Introduction
Cathepsin F ( CTSF) is a member
of the peptidase C1 family. Cathepsins are papain family
cysteine proteinases which are a main component of the lysosomal
proteolytic system. The CTSF gene is ubiquitously
expressed, and it maps to chromosome 11q13, close to the gene encoding
cathepsin W. CTSF plays a role in normal protein catabolism. CTSF involved in some
degradative processes occurring in tumor progression since it is highly
expressed in some cancer cell lines.
Description
CTSF Human Recombinant produced in E.coli is a single,
non-glycosylated polypeptide chain containing 237 amino acids (271-484) and
having a molecular mass of 26kDa.CTSF is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The CTSF solution (1mg/ml) contains 20mM Tris-HCl buffer
(pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSAPPEWDW RSKGAVTKVK DQGMCGSCWA FSVTGNVEGQ WFLNQGTLLS
LSEQELLDCD KMDKACMGGL PSNAYSAIKN LGGLETEDDY SYQGHMQSCN FSAEKAKVYI NDSVELSQNE
QKLAAWLAKR GPISVAINAF GMQFYRHGIS RPLRPLCSPW
LIDHAVLLVG YGNRSDVPFW AIKNSWGTDW GEKGYYYLHR GSGACGVNTM ASSAVVD.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
