Catalogue number
ANT-664
Synonyms
Troponin I cardiac muscle, Cardiac troponin I, TNNI3, TNNC1, CMH7, RCM1, cTnI, CMD2A, MGC116817.
Introduction
Troponin I (TnI), troponin T (TnT) and troponin C (TnC) form the troponin complex of the thin filaments of striated muscle. TnI is acts as the inhibitory subunit by blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains 3 genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TNNI3 gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in the TNNI3 gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
Type
Mouse Anti Human Monoclonal.
Description
TNNI3 Paired antibodies, capture and conjugating are used for lateral flow immunoassay. The sensitivity to detect Cardiac Troponin-I in the blood as declared by the FDA is 5ng/ml. The concentration needed when using the paired antibodies could reach 3-4ng/ml.
We highly recommend using our cat# pro-2266 in the rapid test. The minimal detection quantity of recombinant Cardiac Troponin-I is 0.25 to 0.5ug/ml.
Please note that when ordering for example: 100µg antibody we ship 50µg from each of the antibodies (100µg in total).
Purification Method
Purified monoclonal IgG by protein A chromatography.
Purity
Greater than 95%.
Physical Appearance
2 vials of sterile filtered clear colorless solution.
Formulation
* Cardiac Troponin-I capture antibody in 1x PBS & NaN3.
* Cardiac Troponin-I conjugating antibody in 1x PBS & NaN3.
Stability
Cardiac Troponin-I although stable at 4°C for 1 week, should be stored below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Applications
Lateral flow immunoassay.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
