Catalogue number
PRO-2142
Synonyms
CAP2, PI8, Serpin B8, Cytoplasmic antiproteinase 2, Peptidase
inhibitor 8, CAP-2.
Introduction
Serpin Peptidase Inhibitor, Clade B Member 8 (SERPINB8) belongs to the ov-serpin subfamily, which, compared with the archetypal serpin PI1, is characterized by a high degree of homology to chicken ovalbumin, absence of N- and C-terminal extensions, lack of a signal peptide, and a serine instead of an asparagine residue at the penultimate position. The superfamily of high molecular weight serine proteinase inhibitors (serpins) control various intracellular and extracellular processes such as complement activation, fibrinolysis, coagulation, cellular differentiation, tumor suppression, apoptosis, and cell migration.
Description
SERPINB8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 397 amino acids (1-374 a.a) and having a molecular mass of 45.2kDa.
SERPINB8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SERPINB8 protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMDDLCEA NGTFAISLFK ILGEEDNSRN VFFSPMSISS ALAMVFMGAK
GSTAAQMSQA LCLYKDGDIH RGFQSLLSEV NRTGTQYLLR TANRLFGEKT CDFLPDFKEY CQKFYQAELE
ELSFAEDTEE CRKHINDWVA EKTEGKISEV LDAGTVDPLT KLVLVNAIYF KGKWNEQFDR KYTRGMLFKT
NEEKKTVQMM FKEAKFKMGY ADEVHTQVLE LPYVEEELSM VILLPDDNTD LAVVEKALTY EKFKAWTNSE
KLTKSKVQVF LPRLKLEESY DLEPFLRRLG MIDAFDEAKA DFSGMSTEKN VPLSKVAHKC FVEVNEEGTE
AAAATAVVRN SRCSRMEPRF CADHPFLFFI RHHKTNCILF CGRFSSP.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
