Catalogue number
PRO-446
Synonyms
Avidin, AVD, AVID.
Description
Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons.
Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii.
Advantages
1. Avidin-Biotin does not exhibit unwanted non-specific binding that have been previously reported for the native egg white avidin.
2. The non-specific properties of Avidin-Biotin have been tested in ELISA, Western Blot & Immunohistochemistry techniques and have been found to be superior to unmodified avidin.
3. Avidin-Biotin rarely affected by pH values between the range of pH-2 to pH-12 or by guanidine-HCl concentrations up to 8M at neutral pH.
Physical Appearance
Lyophilized freeze dry Powder.
Formulation
Salt-free lyophilized powder with no preservative added.
Solubility
We highly recommended to prepare a 1 mg/ml solution; Reconstitute using cold deionized pyrogen free water and mix gently until powder is completely dissolved.
Stability
Store protein solution at 2-8°C.
Biological Activity
Binding activity of Avidin-Biotin is 10-15units/mg protein.
One unit of Avidin activity is defined as the amount of protein which will bind 1µg d-biotin.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
